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Journal: Animal Models and Experimental Medicine
Article Title: Baihe Zhimu Decoction ameliorates anxious depression via PI3K / AKT / mTOR pathway‐mediated neuroprotection and inhibition of hippocampal neuronal apoptosis
doi: 10.1002/ame2.70295
Figure Lengend Snippet: Protective effects of BZD‐containing serum against CORT‐induced injury in primary hippocampal neurons. (A) Immunofluorescent co‐localization of NSE (green) and DAPI (blue), scale bar: 50 μm. (B) Cell viability after treatment with different concentrations of BZD‐containing serum for 24 h (MTT assay, n = 6). (C) Protective effects of different concentrations of BZD‐containing serum against CORT‐induced cytotoxicity ( n = 6). (D, E) NeuN staining and quantitative fluorescence analysis. (F–I) Levels of 5‐HT, DA, Glu, and GABA in cell supernatants ( n = 8). Data are presented as mean ± SD. ## p < 0.01 vs . control group; * p < 0.05, ** p < 0.01 vs . model group.
Article Snippet: The neurons were incubated with primary antibodies against NSE (1:300, 66 150‐1‐Ig, Proteintech) and
Techniques: MTT Assay, Staining, Fluorescence, Analysis, Control
Journal: Frontiers in Immunology
Article Title: Case Report: Seronegative rhombencephalitis presenting with ocular flutter and ataxia as a potential sentinel manifestation of gastric adenocarcinoma
doi: 10.3389/fimmu.2026.1844323
Figure Lengend Snippet: Tissue-based assay (TBA) and co-immunostaining of patient serum. Indirect immunofluorescence assay on rat brain tissue sections showing reactivity of patient serum (green), counterstained with DAPI (blue, for cell nuclei) and a commercial anti-NeuN antibody (red, for neuronal nuclei). Rows from top to bottom show representative brain regions: cerebellum, cerebral cortex, hippocampus, and thalamus. Scattered cellular immunoreactivity was observed across all brain regions in the serum sample (green). Merged images (fourth column) demonstrate colocalization of serum IgG reactivity with NeuN-positive neurons, confirming the presence of anti-neuronal antibodies in the serum.
Article Snippet: To confirm neuronal localization, double immunofluorescence was performed using a
Techniques: Tissue, Immunostaining, Immunofluorescence, Blood/Plasma/Serum Samples